Preferential cultivation of type II methanotrophic bacteria from littoral sediments (Lake Constance)
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منابع مشابه
Preferential cultivation of type II methanotrophic bacteria from littoral sediments (Lake Constance).
Most widely used medium for cultivation of methanotrophic bacteria from various environments is that proposed in 1970 by Whittenbury. In order to adapt and optimize medium for culturing of methanotrophs from freshwater sediment, media with varying concentrations of substrates, phosphate, nitrate, and other mineral salts were used to enumerate methanotrophs by the most probable number method. Hi...
متن کاملAbundance and activity of methanotrophic bacteria in littoral and profundal sediments of lake constance (Germany).
The abundances and activities of aerobic methane-oxidizing bacteria (MOB) were compared in depth profiles of littoral and profundal sediments of Lake Constance, Germany. Abundances were determined by quantitative PCR (qPCR) targeting the pmoA gene and by fluorescence in situ hybridization (FISH), and data were compared to methane oxidation rates calculated from high-resolution concentration pro...
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25 Introduction 26 Materials and Methods 28 Results 36 Discussion 47 3. Characterization of a novel methanotroph, Methylosoma difficile gen. nov., sp. nov., enriched by gradient cultivation from littoral sediment of Lake Constance 55 Summary 55 Introduction 57 Methods 58 Results and 64 Discussion 4. Comparison of aerobic methanotrophic communities in littoral and profundal sediment of Lake Cons...
متن کاملActivity and diversity of methanotrophic bacteria at methane seeps in eastern Lake Constance sediments.
The activity and community structure of aerobic methanotrophic communities were investigated at methane seeps (pockmarks) in the littoral and profundal zones of an oligotrophic freshwater lake (Lake Constance, Germany). Measurements of potential methane oxidation rates showed that sediments inside littoral pockmarks are hot spots of methane oxidation. Potential methane oxidation rates at littor...
متن کاملComparison of aerobic methanotrophic communities in littoral and profundal sediments of Lake Constance by a molecular approach.
Analysis of pmoA and 16S rRNA gene clone libraries of methanotrophic bacteria in Lake Constance revealed an overall dominance of type I methanotrophs in both littoral and profundal sediments. The sediments exhibited minor differences in their methanotrophic community structures. Type X methanotrophs made up a significant part of the clone libraries only in the profundal sediment and were also f...
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ژورنال
عنوان ژورنال: FEMS Microbiology Ecology
سال: 2004
ISSN: 0168-6496,1574-6941
DOI: 10.1016/s0168-6496(03)00260-5